Composite

Part:BBa_K3893010:Design

Designed by: Sharon Ortiz   Group: iGEM21_Ecuador   (2021-10-01)


Up part of cassette for dsRNA constitutive production 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 918
    Illegal SpeI site found at 164
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 834


Design Notes

In order to use, test and produce the dsRNA we design before, we created a plasmid backbone with an insert that is able to receive different sequences of dsRNA with a BglII and KpnI digestion.

With this in mind, we face some shortcomings: first, it is a short fragment, with many sequence repeats, which makes it difficult for synthesis. Second, we want this insert to behave as a Biobrick compatible once it has the desired dsRNA sequence cloned inside.

It isn't compatible with RFC10 Assembly. Because the SpeI and BglII restriction sites are needed in the middle of the sequence for the assembly system.


Source

Set of biobricks